2024 PAPERS by Marie Soressi
Quaternary Science Reviews, 2024
The Late Pleistocene archeological record shows emerging patterns of population turnover frequent... more The Late Pleistocene archeological record shows emerging patterns of population turnover frequently associated with technological change between c. 50-40 thousand years ago. In Europe, this is thought to be related to indigenous population admixture and/or the diffusion of developing technologies by Homo sapiens resulting in a widely distributed spatiotemporal patchwork of industries with combinations of Middle and Upper Paleolithic traits. The Late Pleistocene record of Romania forms an anomaly in these scenarios. On the one hand, the country has important Pleistocene archives that preserve direct evidence of early modern humans with Neandertal genetic introgression. On the other hand, Romania shows no evidence of novel technology during the Middle to Upper Paleolithic transition. Here, we review the Late Middle and Early Upper Paleolithic archeological record of Romania supplemented with new radiocarbon ages and excavation data to clarify the validity of this current archeological interpretation. We conclude that while Neandertals and modern humans were in regional contact, raw materials eccentricities and incomplete empirical knowledge of past intergroup cultural transmission have obscured our ability to identify indicative material cultural signals indicating that current methods of understanding hybridized material culture are incomplete.
2023 PAPERS by Marie Soressi
Nature Scientific Reports, 2023
Here we present stable carbon, nitrogen and sulfur isotope ratios of collagen extracted from Rang... more Here we present stable carbon, nitrogen and sulfur isotope ratios of collagen extracted from Rangifer, Equus and Bison bone (n= 128) from diferent stratigraphic levels at the chronologically wellconstrained Middle and Upper Palaeolithic site of Les Cottés, France. Samples were taken from five phases of site use (US08, US06, US04 [upper and lower], and US02; ~ 45.8–35.3 ka cal BP) to explore the dietary and spatial palaeoecology of these ungulate species during MIS 3, and the contemporary climate. Temporal trends in δ15N values of all species broadly align with other climatic indicators at the site and the lowest values in US04 correspond to the Heinrich 4 cooling event, refecting changes in the composition of soil/plant nitrogen at this time. Rangifer collagen is 13C-enriched compared to the other species throughout, consistent with lichen consumption. However, this isotopic niche
partitioning between Rangifer and Equus/Bison is most extensive during US04, indicating plasticity in reindeer feeding behaviour, and potentially overall increased lichen biomass during this cooler/more arid phase. Rangifer δ34S values are consistently lower than Equus and Bison, which could be indicative of their more extensive spatial ranges incorporating greater inland areas. Equus and Bison demonstrate a signifcant decrease in δ34S values through time, which may be linked to contemporary
climatic decline.
Proceedings of the Royal Society B: Biological Sciences, 2023
The narrow-headed vole, collared lemming and common vole were the most abundant small mammal spec... more The narrow-headed vole, collared lemming and common vole were the most abundant small mammal species across the Eurasian Late Pleistocene steppe-tundra environment. Previous ancient DNA studies of the collared lemming and common vole have revealed dynamic population histories shaped by climatic fluctuations. To investigate the extent to which species with similar adaptations share common evolutionary histories, we generated a dataset comprised the mitochondrial genomes of 139 ancient and 6 modern narrow-headed voles from several sites across Europe and northwestern Asia covering approximately the last 100 thousand years (kyr). We inferred Bayesian time-aware phylogenies using 11 radiocarbon-dated samples to calibrate the molecular clock. Divergence of the main mtDNA lineages across the three species occurred during marine isotope stages (MIS) 7 and MIS 5, suggesting a common response of species adapted to open habitat during interglacials. We identified several time-structured mtDNA lineages in European narrow-headed vole, suggesting lineage turnover. The timing of some of these turnovers was synchronous across the three species, allowing us to identify the main drivers of the Late Pleistocene dynamics of steppe- and cold-adapted species.
Archaeological and Anthropological Sciences, 2023
Understanding Palaeolithic hominin subsistence strategies requires the comprehensive taxonomic id... more Understanding Palaeolithic hominin subsistence strategies requires the comprehensive taxonomic identification of faunal remains. The high fragmentation of Late Pleistocene faunal assemblages often prevents proper taxonomic identification based on bone morphology. It has been assumed that the morphologically unidentifiable component of the faunal assemblage would reflect the taxonomic abundances of the morphologically identified portion. In this study, we analyse three faunal datasets covering the Middle to Upper Palaeolithic transition (MUPT) at Bacho Kiro Cave (Bulgaria) and Les Cottés and La Ferrassie (France) with the application of collagen type I peptide mass fingerprinting (ZooMS). Our results emphasise that the fragmented component of Palaeolithic bone assemblages can differ significantly from the morphologically identifiable component. We obtain contrasting identification rates between taxa resulting in an overrepresentation of morphologically identified reindeer (Rangifer tarandus) and an underrepresentation of aurochs/bison (Bos/Bison) and horse/European ass (Equus) at Les Cottés and La Ferrassie. Together with an increase in the relative diversity of the faunal composition, these results have implications for the interpretation of subsistence strategies during a period of possible interaction between Neanderthals and Homo sapiens in Europe. Furthermore, shifts in faunal community composition and in carnivore activity suggest a change in the interaction between humans and carnivores across the MUPT and indicate a possible difference in site use between Neanderthals and Homo sapiens. The combined use of traditional and biomolecular methods allows (zoo)archaeologists to tackle some of the methodological limits commonly faced during the morphological assessment of Palaeolithic bone assemblages.
Nature , 2023
Artefacts made from stones, bones and teeth are fundamental to our understanding of human subsist... more Artefacts made from stones, bones and teeth are fundamental to our understanding of human subsistence strategies, behaviour and culture in the Pleistocene. Although these resources are plentiful, it is impossible to associate artefacts to specific human individuals(1) who can be morphologically or genetically characterized, unless they are found within burials, which are rare in this time period. Thus, our ability to discern the societal roles of Pleistocene individuals based on their biological sex or genetic ancestry is limited(2-5). Here we report the development of a non-destructive method for the gradual release of DNA trapped in ancient bone and tooth artefacts. Application of the method to an Upper Palaeolithic deer tooth pendant from Denisova Cave, Russia, resulted in the recovery of ancient human and deer mitochondrial genomes, which allowed us to estimate the age of the pendant at approximately 19,000-25,000 years. Nuclear DNA analysis identifies the presumed maker or wearer of the pendant as a female individual with strong genetic affinities to a group of Ancient North Eurasian individuals who lived around the same time but were previously found only further east in Siberia. Our work redefines how cultural and genetic records can be linked in prehistoric archaeology.
Rapid Communications in Mass Spectrometry, 2023
Rationale: Proteins extracted from archaeological bone and teeth are utilised for investigating t... more Rationale: Proteins extracted from archaeological bone and teeth are utilised for investigating the phylogeny of extinct and extant species, the biological sex and age of past individuals, as well as ancient health and physiology. However, variable preservation of proteins in archaeological materials represents a major challenge.
Methods: To better understand the spatial distribution of ancient proteins preserved within teeth, we applied matrix assisted laser desorption/ionisation massspectrometry imaging (MALDI-MSI) for the first time to bioarchaeological samples to visualise the intensity of proteins in archaeological teeth thin sections. We specifically explored the spatial distribution of four proteins (collagen type I, of which the chains alpha-1 and alpha-2, alpha-2-HS-glycoprotein, haemoglobin subunit alpha and myosin light polypeptide 6).
Results: We successfully identified ancient proteins in archaeological teeth thin sections using mass spectrometry imaging. The data are available via
ProteomeXchange with identifier PXD038114. However, we observed that peptides did not always follow our hypotheses for their spatial distribution, with distinct differences observed in the spatial distribution of several proteins, and occasionally between peptides of the same protein.
Conclusions: While it remains unclear what causes these differences in protein intensity distribution within teeth, as revealed by MALDI-MSI in this study, we have demonstrated that MALDI-MSI can be successfully applied to mineralised bioarchaeological tissues to detect ancient peptides. In future applications, this technique could be particularly fruitful not just for understanding the preservation of proteins in a range of archaeological materials, but making informed decisions on sampling strategies and the targeting of key proteins of archaeological and biological interest.
2022 PAPERS by Marie Soressi
Nature Scientific Reports, 2022
Recent fossil discoveries suggest that Neandertals and Homo sapiens may have co-existed in Europe... more Recent fossil discoveries suggest that Neandertals and Homo sapiens may have co-existed in Europe for as long as 5 to 6000 years. Yet, evidence for their contemporaneity at any regional scale remains highly elusive. In France and northern Spain, a region which features some of the latest directly-dated Neandertals in Europe, Protoaurignacian assemblages attributed to Homo sapiens appear to 'replace' Neandertal-associated Châtelperronian assemblages. Using the earliest and latest known occurrences as starting points, Bayesian modelling has provided indication that these occupations may in fact have been partly contemporaneous. The reality, however, is that we are unlikely to ever identify the 'first' or 'last' appearance of a species or cultural tradition in the archaeological and fossil record. Here, we use optimal linear estimation modelling to estimate the first appearance date of Homo sapiens and the extinction date of Neandertals in France and northern Spain by statistically inferring these 'missing' portions of the Protoaurignacian and Châtelperronian archaeological records. Additionally, we estimate the extinction date of Neandertals in this region using a dataset of directly-dated Neandertal fossil remains. Our total dataset consists of sixty-six modernly produced radiocarbon determinations which we recalibrated using the newest calibration curve (IntCal20) to produce updated age ranges. The results suggest that the onset of the Homo sapiens occupation of this region likely preceded the extinction of Neandertals and the Châtelperronian by up to 1400-2900 years. This reaffirms the Bayesian-derived duration of coexistence between these groups during the initial Upper Palaeolithic of this region using a novel independent method, and indicates that our understanding of the timing of these occupations may not be suffering from substantial gaps in the record. Whether or not this coexistence featured some form of direct interaction, however, remains to be resolved.
2021 PAPERS by Marie Soressi
Nature, 2021
Nous sommes un groupe d'archéologues, d’anthropologues, de conservateurs et de généticiens de dif... more Nous sommes un groupe d'archéologues, d’anthropologues, de conservateurs et de généticiens de différentes origines et représentant 31 pays. Nous nous sommes rencontrés en novembre 2020 lors d'une table ronde en ligne dédiée à l'éthique de la recherche d’ADN ancien. Nous avons alors convenu de la nécessité de lignes directrices éthiques applicables à l'échelle mondiale. Les recommandations publiées récemment et développées dans le cadre de recherches d’ADN sur des vestiges humains d’Amérique du Nord ne s’avèrent pas généralisables à d’autres régions du monde. Suite à l’analyse de divers contextes, nous proposons ici des lignes directrices que nous pensons être applicables partout dans le monde. Ces lignes directrices sont les suivantes : 1) les chercheurs doivent s'assurer que les règlements s’appliquant dans les lieux où ils travaillent ainsi que dans les lieux d’où proviennent les vestiges humains sont bien respectés ; 2) les chercheurs doivent préparer un programme de recherche détaillé avant de commencer toute étude ; 3) les chercheurs doivent minimiser les dommages causés aux vestiges humains ; 4) les chercheurs doivent s'assurer que les données seront disponibles après la publication pour permettre un réexamen critique des résultats scientifiques; et 5) les chercheurs doivent échanger avec toutes les parties impliquées dès le début de l’étude et respecter leurs points de vue. Nous nous engageons à respecter ces directives et nous souhaitons qu’elles contribuent au renforcement de la pratique éthique de notre champ de recherche.
Nature, 2021
We are a group of archaeologists, anthropologists, curators and geneticists representing diverse ... more We are a group of archaeologists, anthropologists, curators and geneticists representing diverse global communities and 31 countries. All of us met in a virtual workshop dedicated to ethics in ancient DNA research held in November 2020. There was widespread agreement that globally applicable ethical guidelines are needed, but that recent recommendations grounded in discussion about research on human remains from North America are not always generalizable worldwide. Here we propose the following globally applicable guidelines, taking into consideration diverse contexts. These hold that: (1) researchers must ensure that all regulations were followed in the places where they work and from which the human remains derived; (2) researchers must prepare a detailed plan prior to beginning any study; (3) researchers must minimize damage to human remains; (4) researchers must ensure that data are made available following publication to allow critical re-examination of scientifc findings; and (5) researchers must engage with other stakeholders from the beginning of a study and ensure respect and sensitivity to stakeholder perspectives.
We commit to adhering to these guidelines and expect they will promote a high ethical standard in DNA research on human remains going forward.
Journal of Human Evolution, 2021
Genetic and climate-driven estimates of past population dynamics are increasingly influential in ... more Genetic and climate-driven estimates of past population dynamics are increasingly influential in broader models of hominin migration and adaptation, yet the contribution of stone artifact variability remains more contentious. Scientists are increasingly recognizing the potential of unretouched stone flakes ('flakes') in exploring existing models of hominin behavioral evolution. This is because flakes (1) were produced by all stone tool manufacturing groups in the past, (2) are abundant from the inception of the archaeological record up into the ethnographic present, and (3) preserve under most conditions. The statistical tools of 3D geometric morphometrics capture detailed approximations of flake form that are challenging to document with conventional artifact analyses. We analyze a collection of 717 3D scans of experimentally produced flakes from 5 production strategies that were practiced by hominins through large parts of the Pleistocene and that scientists have drawn on also to make demographic arguments about past human behavior (n ¼ 45 reduction sequences, n ¼ 3 knappers naive toward the study objectives). First, as a proof of concept, we demonstrate that we can estimate the strategies used to produce these flakes at a high success rate even when flakes from early stages of core reduction are included. We frame the significance of this finding against archaeological classifications from several key Middle Paleolithic assemblages in France (n ¼ 4 sites, n ¼ 28 layers, n ¼ 16,467 flakes). Second, we show that 3D geometric morphometrics captures subtle differences in these strategies that influence flake formation on a flake-by-flake basis and that reflect decisions made by knappers about platform selection, preparation, and core-surface management. We explore the broader potential of our model with a crossvalidation approach, and we describe a means of assessing flake form on a continuum wherein variability among assemblages separated by large expanses of space and time can be meaningfully explored.
Journal of Archaeological Science: Reports, 2021
Barbed bone points originally deposited in Doggerland are regularly collected from the shores of ... more Barbed bone points originally deposited in Doggerland are regularly collected from the shores of the Netherlands. Their typology and direct 14C dating suggest they are of Mesolithic age. However, the species of which the barbed points were made cannot be identified based on morphological criteria. The bones used to produce the barbed points have been intensively modified during manufacture, use, and post-depositional processes. Here, we taxonomically assess ten barbed points found on the Dutch shore using mass spectrometry and collagen peptide mass fingerprinting alongside newly acquired 14C ages and δ13C and δ15N measurements.
Our results demonstrate a sufficient preservation of unmodified collagen for mass spectrometry-based taxonomic identifications of bone and antler artefacts which have been preserved in marine environments since the beginning of the Holocene. We show that Homo sapiens bones as well as Cervus elaphus bones and antlers were transformed into barbed points. The 14C dating of nine barbed points yielded uncalibrated ages between 9.5 and 7.3 ka 14C BP. The δ13C and δ15N values of the seven cervid bone points fall within the range of herbivores, recovered from the North Sea, whereas the two human bone points indicate a freshwater and/or terrestrial fauna diet.
The wide-scale application of ZooMS is a critical next step towards revealing the selection of species for osseous-tool manufacture in the context of Mesolithic Doggerland, but also further afield. The selection of Cervus elaphus and human bone for manufacturing barbed points in Mesolithic Doggerland is unlikely to have been opportunistic and instead seems to be strategic in nature. Further, the occurrence of Homo sapiens and Cervus elaphus bones in our random and limited dataset suggests that the selection of these species for barbed point production was non-random and subject to specific criteria. By highlighting the transformation of human bones into barbed points – possibly used as weapons – our study provides additional evidence for the complex manipulation of human remains during the Mesolithic, now also evidenced in Doggerland.
Journal of Paleolithic Archaeology, 2021
The recent elaboration and rapid expansion of aDNA, paleoproteomics, and related fields have prop... more The recent elaboration and rapid expansion of aDNA, paleoproteomics, and related fields have propelled a profound "biomolecular turn" in archaeology and fundamentally changed the topology of archaeological knowledge production. Such a transformation of the archaeological research landscape is not without consequence for long-standing research practices in the field, such as lithic analysis. This special issue derives from the session Old Stones, New Eyes? organized by the authors at the UISPP World Congress in Paris in 2018, which aimed to explore the future of lithic studies. An underlying theme of our session was the felt need to respond to the increasing marginalization of lithic research in terms of its capacity to (1) contribute to the grand narratives of early human evolution and (2) better articulate the role and significance of lithic studies in interdisciplinary human origins research. In this editorial, we briefly outline some of the questions and challenges raised by the biomolecular turn and advocate for a more self-conscious and reflexive stance among lithic experts. We argue that lithic studies fulfill all necessary requirements to act as a basic science for human origins research and that its role and status depends less on technological advances, such as, e.g., improved computing facilities, novel analytical software, or automated shape capture technologies, than on continuous work on the conceptual and methodological foundations of inquiry. We finally draw attention to the unique capability of lithic studies to shed light on the human technological condition and illustrate this potential by introducing and briefly discussing the papers included in this issue.
2020 PAPERS by Marie Soressi
Scientific Reports, 2020
Five nearly identical fragments of specialized bone tools, interpreted as lissoirs (French for "s... more Five nearly identical fragments of specialized bone tools, interpreted as lissoirs (French for "smoothers"), have been found at two Middle Paleolithic sites in southwest France. The finds span three separate archaeological deposits, suggesting continuity in the behavior of late Neandertals. Using standard morphological assessments, we determined that the lissoirs were produced on ribs of medium-sized ungulates. However, since these bones are highly fragmented and anthropogenically modified, species determinations were challenging. Also, conservative curation policy recommends minimizing destructive sampling of rare, fragile, or small artifacts for molecular identification methods. To better understand raw material selection for these five lissoirs, we reassess their taxonomy using a non-destructive ZooMS methodology based on triboelectric capture of collagen. We sampled four storage containers and obtained identifiable MALDI-TOF MS collagen fingerprints, all indicative of the same taxonomic clade, which includes aurochs and bison (Bos sp. and Bison sp.). The fifth specimen, which was stored in a plastic bag, provided no useful MALDI-TOF MS spectra. We show that the choice of large bovid ribs in an archaeological layer dominated by reindeer (Rangifer tarandus) demonstrates strategic selection by these Neandertals. Furthermore, our results highlight the value of a promising technique for the non-destructive analysis of bone artifacts.
Applied Sciences, 2020
Archaeological excavation is a demolishing process. Rather few elements outlast extractive opera... more Archaeological excavation is a demolishing process. Rather few elements outlast extractive operations. Therefore, it is hard to visualise the precise location of unearthed finds at a previously excavated research area. Here, we present a mixed reality environment that displays in situ 3D models of features that were formerly extracted and recorded with 3D coordinates during unearthing operations. We created a tablet application that allows the user to view the position, orientation and dimensions of every recorded find while freely moving around the archaeological site with the device.
To anchor the model, we used physical landmarks left at the excavation. A series of customised forms were created to show (onscreen) the different types of features by superimposing them over the terrain as perceived by the tablet camera. The application permits zooming-in, zooming-out, querying for specific artefacts and reading metadata associated with the archaeological elements.
When at the office, our environment enables accurate visualisations of the 3D geometry concerning previously unearthed features and their spatial relationships. The application operates using the Swift programming language, Python scripts and ARKit technology. We present here an example of its use at Les Cottés, France, a palaeolithic site where thousands of artefacts are excavated out of six superimposed layers with a complex conformation.
Archaeological and Anthropological Sciences, 2020
Increasingly researchers have employed confocal microscopy and 3D surface texture analysis to ass... more Increasingly researchers have employed confocal microscopy and 3D surface texture analysis to assess bone surface modifications in an effort to understand ancient behavior. However, quantitative comparisons between the surfaces of purported archaeological bone tools and experimentally manufactured and used bones are complicated by taphonomic processes affecting ancient bone. Nonetheless, it may be reasonable to assume that bones within the same deposits are altered similarly and thus these alterations are quantifiable. Here we show how unworked bones can be used to quantify the taphonomic effect on bone surfaces and how this effect can then be controlled for and incorporated into an analysis for evaluating the modified surfaces of purported bone tools. To assess the baseline taphonomy of Middle Paleolithic archaeological deposits associated with typologically identified bone artifacts, specifically lissoirs, we directly compare the surface textures of ancient and modern unworked ribs. We then compare the ancient unworked ribs and lissoirs to assess their differences and predict the ancient artifacts' original surface state using a multilevel multivariate Bayesian model. Our findings demonstrate that three of five tested surface texture parameters (Sa, Spc, and IsT) are useful for distinguishing surface type. Our model predictions show that lissoirs tend to be less rough, have more rounded surface peaks, and exhibit more directionally oriented surfaces. These characteristics are likely due to anthropogenic modifications and would have been more pronounced at deposition. Quantifying taphonomic alterations moves us one step closer to accurately assessing how bone artifacts were made and used in the ancient past.
2019 PAPERS by Marie Soressi
Scientific Reports, 2019
The transition from Middle to Upper Paleolithic is a major biological and cultural threshold in t... more The transition from Middle to Upper Paleolithic is a major biological and cultural threshold in the construction of our common humanity. Technological and behavioral changes happened simultaneously to a major climatic cooling, which reached its acme with the Heinrich 4 event, forcing the human populations to develop new strategies for the exploitation of their environment. The recent fieldwork at Les Cottés (France) transitional site offers a good opportunity to document subsistence strategies for this period and to provide for the first time high-resolution insights on its evolution. We present the results of the complete zooarchaeological and taphonomic analysis of the transitional sequence, associated with a large regional synthesis of the subsistence strategy evolution during the Middle to Upper Paleolithic. We conclude that, while there is no major change in the hunting strategies, the butchery activities evolved in strict correlation with the development of range weapons. In addition, the demise of carnivore seems to be a consequence of the human pressure on the environment. Our study demonstrates how the faunal component of the environment became a structuring element of the human social organization, being at the base of future cultural evolutions.
Exceptionally high δ15N values in collagen single amino acids confirm Neandertals as high-trophic level carnivores
PNAS, Mar 12, 2019
Isotope and archeological analyses of Paleolithic food webs have suggested that Neandertal subsis... more Isotope and archeological analyses of Paleolithic food webs have suggested that Neandertal subsistence relied mainly on the consumption of large herbivores. This conclusion was primarily based on elevated nitrogen isotope ratios in Neandertal bone collagen and has been significantly debated. This discussion relies on the observation that similar high nitrogen isotopes values could also be the result of the consumption of mammoths, young animals, putrid meat, cooked food, freshwater fish, carnivores, or mushrooms. Recently, compound-specific C and N isotope analyses of bone collagen amino acids have been demonstrated to add significantly more information about trophic levels and aquatic food consumption. We undertook single amino acid C and N isotope analysis on two Neandertals, which were characterized by exceptionally high N isotope ratios in their bulk bone or tooth collagen. We report here both C and N isotope ratios on single amino acids of collagen samples for these two Neandertals and associated fauna. The samples come from two sites dating to the Middle to Upper Paleolithic transition period (Les Cottés and Grotte du Renne, France). Our results reinforce the interpretation of Neandertal dietary adaptations as successful top-level carnivores, even after the arrival of modern humans in Europe. They also demonstrate that high δ15N values of bone collagen can solely be explained by mammal meat consumption, as supported by archeological and zooarcheological evidence, without necessarily invoking explanations including the processing of food (cooking, fermenting), the consumption of mammoths or young mammals, or additional (freshwater fish, mushrooms) dietary protein sources.
Journal of Computer Applications in Archaeology, 2019
This study uses data extracted from 3D models to compare blade cores from the Châtelperronian and... more This study uses data extracted from 3D models to compare blade cores from the Châtelperronian and Protoaurignacian stone tool industries. These technocomplexes are at the center of the debate surrounding the interactions between Neanderthals and anatomically modern humans approximately 45 to 40,000 years ago.
We created 3D models of lithic cores from the sites of Roc de Combe and Les Cottés using a standardized photogrammetry protocol. We then used data derived from these 3D models to make quantitative comparisons of artifact attributes that have previously been argued to distinguish the two stone tool industries in question. These attributes include the angle between the platform and flaking surfaces, the shape of core cross sections, and the angle between core axes. The conception of this study was not to privilege the use of new technological and statistical approaches over more traditional or qualitative forms of lithic analysis. Rather, our aim was to experiment with using digital tool to develop nuanced, reproducible ways to describe variability in lithic artifacts.
Our results support the hypothesis that there is a difference in the angle between core surfaces between these two industries. Our analysis also indicates a difference in the angle between core axes, although we are more cautious in interpreting these results. An elliptical Fourier analysis of core cross section shape was inconclusive. We discuss what archaeological and methodological factors may have contributed to our results, and the roles of both qualitative and quantitative observations in archaeological research. 3D artifact models generated for this study are included as supplemental data and are available for use by other researchers.
2018 PAPERS by Marie Soressi
Reconstructing the genetic history of late Neanderthals
Nature, 2018
Although it has previously been shown that Neanderthals contributed DNA to modern humans, not muc... more Although it has previously been shown that Neanderthals contributed DNA to modern humans, not much is known about the genetic diversity of Neanderthals or the relationship between late Neanderthal populations at the time at which their last interactions with early modern humans occurred and before they eventually disappeared. Our ability to retrieve DNA from a larger number of Neanderthal individuals has been limited by poor preservation of endogenous DNA and contamination of Neanderthal skeletal remains by large amounts of microbial and present-day human DNA. Here we use hypochlorite treatment of as little as 9 mg of bone or tooth powder to generate between 1- and 2.7-fold genomic coverage of five Neanderthals who lived around 39,000 to 47,000 years ago (that is, late Neanderthals), thereby doubling the number of Neanderthals for which genome sequences are available. Genetic similarity among late Neanderthals is well predicted by their geographical location, and comparison to the genome of an older Neanderthal from the Caucasus indicates that a population turnover is likely to have occurred, either in the Caucasus or throughout Europe, towards the end of Neanderthal history. We find that the bulk of Neanderthal gene flow into early modern humans originated from one or more source populations that diverged from the Neanderthals that were studied here at least 70,000 years ago, but after they split from a previously sequenced Neanderthal from Siberia around 150,000 years ago. Although four of the Neanderthals studied here post-date the putative arrival of early modern humans into Europe, we do not detect any recent gene flow from early modern humans in their ancestry.
The recently renovated Mus ee de l'Homme in Paris is currently hosting an exhibition on Neanderth... more The recently renovated Mus ee de l'Homme in Paris is currently hosting an exhibition on Neanderthals. Running from March 2018 to January 2019, N eandertal (in the singular form) presents original finds from excavations all over Europe alongside nineteenth and twentieth century artworks to discuss the rehabilitation of the image of the Neanderthal as a human species in its own right ). In a nutshell, the message of the exhibition is "Neanderthals were people, too". This message is well-delivered and the visitor leaves the show having learned about the many aspects of Neanderthals' craft, life, and destiny. Given the recent advances in human evolution studies, we wonder if it would have been possible to place more emphasis on past human diversity echoing in some ways the theme of the permanent exhibition of the Mus ee de l'Homme.
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2024 PAPERS by Marie Soressi
2023 PAPERS by Marie Soressi
partitioning between Rangifer and Equus/Bison is most extensive during US04, indicating plasticity in reindeer feeding behaviour, and potentially overall increased lichen biomass during this cooler/more arid phase. Rangifer δ34S values are consistently lower than Equus and Bison, which could be indicative of their more extensive spatial ranges incorporating greater inland areas. Equus and Bison demonstrate a signifcant decrease in δ34S values through time, which may be linked to contemporary
climatic decline.
Methods: To better understand the spatial distribution of ancient proteins preserved within teeth, we applied matrix assisted laser desorption/ionisation massspectrometry imaging (MALDI-MSI) for the first time to bioarchaeological samples to visualise the intensity of proteins in archaeological teeth thin sections. We specifically explored the spatial distribution of four proteins (collagen type I, of which the chains alpha-1 and alpha-2, alpha-2-HS-glycoprotein, haemoglobin subunit alpha and myosin light polypeptide 6).
Results: We successfully identified ancient proteins in archaeological teeth thin sections using mass spectrometry imaging. The data are available via
ProteomeXchange with identifier PXD038114. However, we observed that peptides did not always follow our hypotheses for their spatial distribution, with distinct differences observed in the spatial distribution of several proteins, and occasionally between peptides of the same protein.
Conclusions: While it remains unclear what causes these differences in protein intensity distribution within teeth, as revealed by MALDI-MSI in this study, we have demonstrated that MALDI-MSI can be successfully applied to mineralised bioarchaeological tissues to detect ancient peptides. In future applications, this technique could be particularly fruitful not just for understanding the preservation of proteins in a range of archaeological materials, but making informed decisions on sampling strategies and the targeting of key proteins of archaeological and biological interest.
2022 PAPERS by Marie Soressi
2021 PAPERS by Marie Soressi
We commit to adhering to these guidelines and expect they will promote a high ethical standard in DNA research on human remains going forward.
Our results demonstrate a sufficient preservation of unmodified collagen for mass spectrometry-based taxonomic identifications of bone and antler artefacts which have been preserved in marine environments since the beginning of the Holocene. We show that Homo sapiens bones as well as Cervus elaphus bones and antlers were transformed into barbed points. The 14C dating of nine barbed points yielded uncalibrated ages between 9.5 and 7.3 ka 14C BP. The δ13C and δ15N values of the seven cervid bone points fall within the range of herbivores, recovered from the North Sea, whereas the two human bone points indicate a freshwater and/or terrestrial fauna diet.
The wide-scale application of ZooMS is a critical next step towards revealing the selection of species for osseous-tool manufacture in the context of Mesolithic Doggerland, but also further afield. The selection of Cervus elaphus and human bone for manufacturing barbed points in Mesolithic Doggerland is unlikely to have been opportunistic and instead seems to be strategic in nature. Further, the occurrence of Homo sapiens and Cervus elaphus bones in our random and limited dataset suggests that the selection of these species for barbed point production was non-random and subject to specific criteria. By highlighting the transformation of human bones into barbed points – possibly used as weapons – our study provides additional evidence for the complex manipulation of human remains during the Mesolithic, now also evidenced in Doggerland.
2020 PAPERS by Marie Soressi
To anchor the model, we used physical landmarks left at the excavation. A series of customised forms were created to show (onscreen) the different types of features by superimposing them over the terrain as perceived by the tablet camera. The application permits zooming-in, zooming-out, querying for specific artefacts and reading metadata associated with the archaeological elements.
When at the office, our environment enables accurate visualisations of the 3D geometry concerning previously unearthed features and their spatial relationships. The application operates using the Swift programming language, Python scripts and ARKit technology. We present here an example of its use at Les Cottés, France, a palaeolithic site where thousands of artefacts are excavated out of six superimposed layers with a complex conformation.
2019 PAPERS by Marie Soressi
We created 3D models of lithic cores from the sites of Roc de Combe and Les Cottés using a standardized photogrammetry protocol. We then used data derived from these 3D models to make quantitative comparisons of artifact attributes that have previously been argued to distinguish the two stone tool industries in question. These attributes include the angle between the platform and flaking surfaces, the shape of core cross sections, and the angle between core axes. The conception of this study was not to privilege the use of new technological and statistical approaches over more traditional or qualitative forms of lithic analysis. Rather, our aim was to experiment with using digital tool to develop nuanced, reproducible ways to describe variability in lithic artifacts.
Our results support the hypothesis that there is a difference in the angle between core surfaces between these two industries. Our analysis also indicates a difference in the angle between core axes, although we are more cautious in interpreting these results. An elliptical Fourier analysis of core cross section shape was inconclusive. We discuss what archaeological and methodological factors may have contributed to our results, and the roles of both qualitative and quantitative observations in archaeological research. 3D artifact models generated for this study are included as supplemental data and are available for use by other researchers.
2018 PAPERS by Marie Soressi